Analytical Studies and Biological Activity of New Azo Dye and Its Complexation with Zinc

Authors

  • Ali Kamil Mohsin College of Education for Pure Sciences, University of Basrah - Iraq
  • Hanan M. Ali College of Education for Pure Sciences, University of Basrah - Iraq

DOI:

https://doi.org/10.48112/bcs.v2i1.324

Abstract

Abstract Views: 194

Azo dye (AD), (Z)-5-amino-2-(((4-(5-(p-tolyl)-3-(trifluoromethyl)-1H-pyrazol-1-yl) phenyl) sulfonyl) diazenyl) phenol) and its complex with zinc were prepared and characterized using FTIR, UV-visible, elemental analysis and mass spectrum. Analytical studies showed that this dye had high solubility in ethanol and gave three isopiestic points when studying the pH effect in a range of buffer solutions. The protonation constants pKb1 and pKb2 of nitrogen atom and the ionization constant pKa of OH-group were equal to 3.4, 7.26 and 10.5 respectively. These results indicated the suggested ionization scheme in acidic and basic media. Further, the synthetic AD was provided non-toxic effects using different concentrations, and did not show any heamolysis effects in the cells in contrast with another chemical compound. The new AD is considered as novel medicine or chemical sanitizer. Further, the biological activity of AD and its complex was tested against four different bacteria, (Staphylococcus Aureus, Escherichia Coli, Bacillus Cereus and Pseudomonas Aeruginosa) and two different fungi (Aspergillus Albicans and Candida Albicans). The results showed that the complexation of AD with zinc was decreased the biological activity of synthetic AD.

Keywords:

Azo dye, Biological activity, Elemental analysis, Haemolysis effects, Ionization constants

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References

Ali, A. A., Fahad, T. A., & Al-muhsin, A. A. (2020, November). Preparation and Spectroanalytical Studies of Two New Azo Dyes Based on Luminol. In IOP Conference Series: Materials Science and Engineering (Vol. 928, No. 5, p. 052007). IOP Publishing. https://doi.org/10.1088/1757-899x/928/5/052007

Ali, H., Majeed, H., & Hussain, A. (2017). Synthesis, Analytical and Theoretical studies of (Z)-4amino-3-hydroxy-2-((4-(N-(5-methyl isoxazol-3yl) sulfamoyl) phenyl) diazenyl) naphthalene-1-Sulfonic Acid. Journal of Natural Sciences Research, 7, 81-88.

Ali, H., Majeed, H. A. S. A., Al-Asadi, I., Abdulredha, A., & Hussain, A. (2018). Structures effect of two azo dyes associated with their antimicrobial activity. Journal of Chemical, Biological and Physical Sciences, 8, 171-185.

Ali H., Majeed H., Hussain A. and Mohsin R., Journal of Global Pharma Technology Analytical and Theoretical Studies of Some Diazo Dyes, Journal of Global Pharma Technology, 2019 11, 353-362.

Eady, S. J., Ali, H. M., & Al-Shawi, A. A. (2018). Preparation of novel Azo Dyes as a new anti-Human Breast Cancer MDA-MB231 Cells and study its association with DNA. Journal of Basrah Researches (Sciences), 29, 1-11.

Kirkan, B., & Gup, R. (2008). Synthesis of new azo dyes and copper (II) complexes derived from barbituric acid and 4-aminobenzoylhydrazone. Turkish Journal of Chemistry, 32(1), 9-17. https://journals.tubitak.gov.tr/chem/vol32/iss1/2

Majeed, H. (2013). Synthesis, Characterization, and study of the Spectral and Electronic Properties of a New Azo Dyes Compounds. University of Thi-Qar Journal of Science, 4(1), 91-101.

Farghaly, T. A., & Abdallah, Z. A. (2008). Synthesis, azo-hydrazone tautomerism and antitumor screening of N-(3-ethoxycarbonyl-4, 5, 6, 7-tetrahydro-benzo [b] thien-2-yl)-2-arylhydrazono-3-oxobutanamide derivatives. Arkivoc, 17, 295-305.

Fayadh, R. H., Ali, A. A., & Al Jabri, F. M. (2015). Synthesis and identification symmetrically azo dyes derived from sulfa compounds and spectrophotometric study of nickel (II) complexes with prepared dyes. International Journal of Engineering and Technical Research (IJETR), 3, 25-28.

Fayadh R., Ali A. and Al – Jabri F., The Synthesis and Identification Azo dyes Derived from Mercuried Sulfa, compounds and used their as Indicator of Acid – Base, Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2015, 6, 1278-1285.

Fox J., J. Chem. Soc. (1910), 97: 1339 (b) Majeed H., Al-Ahmad A., Hussain K., The Preparation, Characterization and the Study of the Linear Optical Properties.

Issa, R. M., & Zewail, A. H. (1971). Spectrophotometric studies on purpurin and quinalizarin in aqueous solutions. United Arab Republic Journal of Chemistry, 14(5), 461-472. https://authors.library.caltech.edu/78811/

Mutar, M. M., & Ali, H. M. (2021, November). Preparation, Characterization and Analytical Studies of novel Azo dyes and Diazo dyes. In Journal of Physics: Conference Series (Vol. 2063, No. 1, p. 012023). IOP Publishing. https://doi.org/0.1088/1742-6596/2063/1/012023

Otutu, J. O. (2013). Synthesis and application of azo dyes derived from 2-Amino-1, 3, 4-thiadiazole-2-thiol on polyester fibre. International Journal of Research and Reviews in Applied Sciences, 15(2), 292-296. https://www.cabdirect.org/cabdirect/abstract/20133333946

Primo, F. T., & Fröhlich, P. E. (2005). Celecoxib identification methods. Acta Farmaceutica Bonaerense, 24(3), 421.

Thangavelu, R., Devi, P. G., Gopi, M., & Mustaffa, M. M. (2013). Management of Eumusae leaf spot disease of banana caused by Mycosphaerella eumusae with Zimmu (Allium sativum× Allium cepa) leaf extract. Crop protection, 46, 100-105. https://doi.org/10.1016/j.cropro.2012.12.022

Xian-Guo, H., & Ursula, M. (1994). Determination of hemolysis against fresh sheep RBCs. J. Ethnopharmacol, 43, 173-177.

Analytical Studies and Biological Activity of New Azo Dye and Its Complexation with Zinc

Published

2023-01-01

How to Cite

Mohsin, A. K., & Ali, H. M. (2023). Analytical Studies and Biological Activity of New Azo Dye and Its Complexation with Zinc. Biomedicine and Chemical Sciences, 2(1), 31–38. https://doi.org/10.48112/bcs.v2i1.324

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Articles