Assessment of Antibacterial Activity in Vitro
Eco-Friendly Synthesis and Characterization of Silver Nanoparticles
DOI:
https://doi.org/10.48112/bcs.v2i3.556Abstract
Abstract Views: 610
Silver nanoparticles (AgNPs) are one of the most essential and interesting nano materials between different metallic nanoparticles that are implicated in biomedical utilization. The expansion number of resistant bacteria create an inquiry for new antibiotic methods. Metallic nanoparticles have derived as a new platform against different microorganisms. The nanoparticles can by oxidative stress damaging the membrane of bacteria and DNA. Synthesis novel sliver nanoparticles using different reducing and stabilizing agents and Study antibacterial activity of the synthesized silver nanoparticles. Silver nanoparticles (AgNPs) were synthesis by utilizing green, simple and easy approach chemical reduction method using glucose as reducing and gelatin as stabilizing agent. The optimum conditions of AgNPs synthesizing were obtained by varying the concentration of AgNO3, concentration ratio of glucose /AgNO3 and temperature. The synthesized AgNPs were determined by UV—vis spectrum. Atomic Force Microscopy (AFM), Scanning electron microscopy (SEM), and Fourier transmission infrared spectroscopy (FTIR) analysis. Chemistry method for producing. The obtained AgNPs with particle size 75.7nm. Silver nanoparticles (AgNPs) showed excellent antibacterial against Gram -negative bacterial strains Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Gram-positive bacteria, Staphylococcus aureus, Bacillus cereus.
Keywords:
Biological application, Silver nanoparticles, Synthesis of nanoparticlesReferences
Ali, A. R., Anani, H. A., & Selim, F. M. (2021). Biologically formed silver nanoparticles and in vitro study of their antimicrobial activities on resistant pathogens. Iranian Journal of Microbiology, 13(6), 848. https://doi.org/10.18502%2Fijm.v13i6.8090
Al-Nimry, S., Dayah, A. A., Hasan, I., & Daghmash, R. (2021). Cosmetic, biomedical and pharmaceutical applications of fish gelatin/hydrolysates. Marine drugs, 19(3), 145. https://doi.org/10.3390/md19030145
Baig, N., Kammakakam, I., & Falath, W. (2021). Nanomaterials: A review of synthesis methods, properties, recent progress, and challenges. Materials Advances, 2(6), 1821-1871. https://doi.org/10.1039/D0MA00807A
Bayda, S., Adeel, M., Tuccinardi, T., Cordani, M., & Rizzolio, F. (2019). The history of nanoscience and nanotechnology: from chemical–physical applications to nanomedicine. Molecules, 25(1), 112. https://doi.org/10.3390/molecules25010112
Darroudi, M., Ahmad, M. B., Abdullah, A. H., & Ibrahim, N. A. (2011). Green synthesis and characterization of gelatin-based and sugar-reduced silver nanoparticles. International Journal of nanomedicine, 569-574. https://doi.org/10.2147%2FIJN.S16867
Gamboa, S. M., Rojas, E. R., Martínez, V. V., & Vega-Baudrit, J. (2019). Synthesis and characterization of silver nanoparticles and their application as an antibacterial agent. Int. J. Biosen. Bioelectron, 5, 166-173. https://doi.org/10.15406/ijbsbe.2019.05.00172
Iravani, S., Korbekandi, H., Mirmohammadi, S. V., & Zolfaghari, B. (2014). Synthesis of silver nanoparticles: chemical, physical and biological methods. Research in pharmaceutical sciences, 9(6), 385-406. https://pubmed.ncbi.nlm.nih.gov/26339255
Jacob Inbaneson, S., Ravikumar, S., & Manikandan, N. (2011). Antibacterial potential of silver nanoparticles against isolated urinary tract infectious bacterial pathogens. Applied Nanoscience, 1, 231-236. https://doi.org/10.1007/s13204-011-0031-2
Khan, N., Kumar, D., & Kumar, P. (2020). Silver nanoparticles embedded guar gum/gelatin nanocomposite: green synthesis, characterization and antibacterial activity. Colloid and Interface Science Communications, 35, 100242. https://doi.org/10.1016/j.colcom.2020.100242
Khanh, L. L., Truc, N. T., Dat, N. T., Nghi, N. T. P., Van Toi, V., Hoai, N. T. T., ... & Hiep, N. T. (2019). Gelatin-stabilized composites of silver nanoparticles and curcumin: characterization, antibacterial and antioxidant study. Science and Technology of Advanced Materials, 20(1), 276–290. https://doi.org/10.1080%2F14686996.2019.1585131
Mees, C., & James, F. K. (1966). The theory of the photographic process. New York: MacMillan.
Mikhailov, O. V. (2023). Gelatin as It Is: History and Modernity. International Journal of Molecular Sciences, 24(4), 3583. https://doi.org/10.3390/ijms24043583
Peng, S., McMahon, J. M., Schatz, G. C., Gray, S. K., & Sun, Y. (2010). Reversing the size-dependence of surface plasmon resonances. Proceedings of the National Academy of Sciences, 107(33), 14530-14534. https://doi.org/10.1073/pnas.1007524107
Platania, V., Kaldeli-Kerou, A., Karamanidou, T., Kouki, M., Tsouknidas, A., & Chatzinikolaidou, M. (2021). Antibacterial effect of colloidal suspensions varying in silver nanoparticles and ions concentrations. Nanomaterials, 12(1), 31. https://doi.org/10.3390/nano12010031
Samuel, M. S., Ravikumar, M., John J, A., Selvarajan, E., Patel, H., Chander, P. S., ... & Chandrasekar, N. (2022). A review on green synthesis of nanoparticles and their diverse biomedical and environmental applications. Catalysts, 12(5), 459. https://doi.org/10.3390/catal12050459
Vanaja, M., Paulkumar, K., Baburaja, M., Rajeshkumar, S., Gnanajobitha, G., Malarkodi, C., ... & Annadurai, G. (2014). Degradation of methylene blue using biologically synthesized silver nanoparticles. Bioinorganic Chemistry and Applications, 2014. https://doi.org/10.1155/2014/742346
Vishwanath, R., & Negi, B. (2021). Conventional and green methods of synthesis of silver nanoparticles and their antimicrobial properties. Current Research in Green and Sustainable Chemistry, 4, 100205. https://doi.org/10.1016/j.crgsc.2021.100205
Ward, A. G., & Courts, A. (1977). The science and technology of gelatin. London: Academic Press; 1977.
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