Evaluating the Inhibitory Effect of Streptomyces Bacteria against Pathogenic Bacteria and Study its Compatibility with Some Antibiotic Types


  • Mohsen Hashem Risan College of Biotechnology, Al-Nahrain University, Baghdad – Iraq https://orcid.org/0000-0001-5574-9156
  • Shams Ahmed Subhi Al-Turath University College –Iraq
  • Baydaa Y Hussain College of Biotechnology, Al-Nahrain University, Baghdad – Iraq




Abstract Views: 196

All Streptomyces sp isolates were screened for their antibacterial activity on Yeast extract-malt extract agar medium (ISP2) using scross-streak technique against two pathogenic bacteria include Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus. Among three Streptomyces sp isolates that obtained from Baghdad city (Al-Jadriya), one isolates (B2) didn’t show any antibacterial activity against any type of pathogenic bacteria (Gram-negative and Gram-positive bacteria), while two Streptomyces sp isolates (B1 and B3) showed antibacterial activity against Gram Two-negative (Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus). Screening was performed by Agar-Well Diffusion method and growth inhibition zones were measured in millimeters for each of the Streptomyces isolates (B1 and B3). Tested isolates have shown potent in vitro antibacterial activities against all tested pathogens.  The highest activities were shown by isolate B1 against S. aureus 19.5 mm, Pseudomonas aeruginosa 14 mm. It is also evident that B3 isolate has shown activities against all pathogenic bacteria with inhibition zone diameters ranging between 17 and 13 mm against S. aureus and P. aeruginosa respectively. The effects of Levofloxacin, Sulfamethoxazole, Ciprofloxacin, Ceftriaxone, Aztreonam, Amikacin and Gentamicin on growth of Streptomyces sp were evaluated over a 48 h period. Morphology and growth of Streptomyces sp. were not affected by all antibiotics, all Streptomyces isolates (B1, B2) were screened for resistance against seven antibiotics, all Streptomyces isolates were resistance against all antibiotics.


Streptomyces, Bacteria, Compatibility, Antibiotics


Metrics Loading ...


Al-rubaye, T. S., Risan, M. H., & Al-Rubaye, D. (2020). Gas chromatography-mass-spectroscopy analysis of bioactive compounds from Streptomyces spp. isolated from Tigris river sediments in Baghdad city. Journal of Biotechnology Research Center, 14(1), 63-71. https://doi.org/10.24126/jobrc.2020.14.1.590

Al-Rubaye, T. S., Risan, M. H., Al-Rubaye, D., & Radi, R. O. (2018). Characterization of marine Streptomyces spp. bacterial isolates from Tigris river sediments in Baghdad city with Lc-ms and 1 HNMR. Journal of Pharmacognosy and Phytochemistry, 7(5), 2053-2060.

Amin, S. M., Rasin, M. H., & Abdulmohimin, N. (2016). Antimicrobial and Antioxidant Activities of Biologically Active Extract from Locally Isolated Actinomycetes in Garmian Area. Journal of Garmian University, 1(10). 625-639.

Ashelford, K. E., Day, M. J., & Fry, J. C. (2003). Elevated abundance of bacteriophage infecting bacteria in soil. Applied and environmental microbiology, 69(1), 285-289. https://doi.org/10.1128/AEM.69.1.285-289.2003

Berdy, J. (2005). Bioactive microbial metabolites. The Journal of antibiotics, 58(1), 1-26. https://doi.org/10.1038/ja.2005.1

Collins, C. H.; Lyne, P. M. and Grange J. M. (1995). Microbiological methods. 7th edition, Butterworth Heinemann Ltd. London.

Cowan, S. T. (1974). Cowan and Steel's Manual for the Identification of Medical Bacteria, 2nd Edn. London: Cambridge University Press.

Czárán, T. L., Hoekstra, R. F., & Pagie, L. (2002). Chemical warfare between microbes promotes biodiversity. Proceedings of the National Academy of Sciences, 99(2), 786-790. https://doi.org/10.1073/pnas.012399899

Deshmukh, A. (1997): Hand book of media, stains and reagents in microbiology. PAMA publication. 14.

Gesheva, V., Ivanova, V., & Gesheva, R. (2005). Effects of nutrients on the production of AK-111-81 macrolide antibiotic by Streptomyces hygroscopicus. Microbiological research, 160(3), 243-248. https://doi.org/10.1016/j.micres.2004.06.005

Goodfellow et al., 1988. Actinomycetes in Biotechnology. Academic Press, London.

Hopwood D. A. (2007). Actinomycetes and Antibiotics; Antibiotic Discovery and Resistance. Streptomyces in Nature and Medicine: The Antibiotic Makers. Oxford University Press, New York, NY. pp. 8-50.

Kariminik, A., & Baniasadi, F. (2010). Pageantagonistic activity of Actinomycetes on some Gram negative and Gram positive bacteria. World Applied Sciences Journal, 8(7), 828-832.

Kieser et al., 2000. General introduction to actinomycete biology. Practical Streptomyces Genetics (2nd ed.). John Innes Foundation, Norwich, England. pp. 2-41.

Kinkel, L. L., Schlatter, D. C., Xiao, K., & Baines, A. D. (2014). Sympatric inhibition and niche differentiation suggest alternative coevolutionary trajectories among Streptomycetes. The ISME journal, 8(2), 249-256. https://doi.org/10.1038/ismej.2013.175

Martinez, J. L., Sánchez, M. B., Martínez-Solano, L., Hernandez, A., Garmendia, L., Fajardo, A., & Alvarez-Ortega, C. (2009). Functional role of bacterial multidrug efflux pumps in microbial natural ecosystems. FEMS microbiology reviews, 33(2), 430-449. https://doi.org/10.1111/j.1574-6976.2008.00157.x

Oskay, A. M., Üsame, T., & Cem, A. (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African journal of Biotechnology, 3(9), 441-446. https://doi.org/10.5897/AJB2004.000-2087

Pandey, B., Ghimire, P., & Agrawal, V. P. (2004). Studies on the antibacterial activity of the Actinomycetes isolated from the Khumbu Region of Nepal. Journal Biology Science, 23, 44-53.

Portillo, M. C., Saiz-Jimenez, C., & Gonzalez, J. M. (2009). Molecular characterization of total and metabolically active bacterial communities of “white colonizations” in the Altamira Cave, Spain. Research in microbiology, 160(1), 41-47. https://doi.org/10.1016/j.resmic.2008.10.002

Qasim, B., & Risan, M. H. (2017). Anti-tumor and Antimicrobial Activity of Antibiotic Produced by Streptomyces spp. World Journal of Pharmaceutical Research, 6(4), 116-128.

Ramazani, A., Moradi, S., Sorouri, R., Javani, S., & Garshasbi, M. (2013). Screening for antibacterial activity of Streptomyces species isolated from Zanjan province, Iran. Int J Pharm Chem Biol Sci, 3(2), 342-349.

Risan, M. H., Jafar, R. A., & Subhi, S. A. (2019). Isolation, characterization and antibacterial activity of a Rare Actinomycete: Saccharopolyspora sp. In Iraq. East African Scholars Journal of Biotechnology and Genetics, 1(4), 60-49.

Risan, M. H., Qasim, B., Abdel-jabbar, B., & Muhsin, A. H. (2017). Identification Active Compounds of Bacteria Streptomyces Using High-Performance Liquid Chromatography. World Journal of Pharmaceutical and Life Sciences, 3(6), 91-97.

Saadoun, I., Al-Joubori, B., & Al-Khoury, R. (2015). Testing of production of inhibitory bioactive compounds by soil Streptomycetes as preliminary screening programs in UAE for anti-cancer and anti-bacterial drugs. Int. J. Curr. Microbiol. App. Sci, 4(3), 446-459.

Stackebrandt, E., & GOEBEL, B. M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International journal of systematic and evolutionary microbiology, 44(4), 846-849.

Stackebrandt, E., Rainey, F. A., & Ward-Rainey, N. L. (1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. International Journal of Systematic and Evolutionary Microbiology, 47(2), 479-491. https://doi.org/10.1099/00207713-47-2-479

Thompson J. N. (2005) The Geographic Mosaic Of Coevolution. University of Chicago Press, Chicago.

Vaz Jauri, P., Bakker, M. G., Salomon, C. E., & Kinkel, L. L. (2013). Subinhibitory antibiotic concentrations mediate nutrient use and competition among soil Streptomyces. PLoS One, 8(12), e81064. https://doi.org/10.1371/journal.pone.0081064

Vimal, V., Rajan, B. M., & Kannabiran, K. (2009). Antimicrobial activity of marine actinomycete, Nocardiopsis sp. VITSVK 5 (FJ973467). Asian J Med Sci, 1(2), 57-63.

Weekers, P. H., Bodelier, P. L., Wijen, J. P., & Vogels, G. D. (1993). Effects of grazing by the free-living soil amoebae Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis on various bacteria. Applied and Environmental Microbiology, 59(7), 2317-2319. https://doi.org/10.1128/aem.59.7.2317-2319.1993

Wiener, P., Egan, S., & Wellington, E. M. H. (1998). Evidence for transfer of antibiotic‐resistance genes in soil populations of streptomycetes. Molecular ecology, 7(9), 1205-1216. https://doi.org/10.1046/j.1365-294x.1998.00450.x

Yim, G., Huimi Wang, H., & Davies Frs, J. (2007). Antibiotics as signalling molecules. Philosophical Transactions of the Royal Society B: Biological Sciences, 362(1483), 1195-1200. https://doi.org/10.1098/rstb.2007.2044

Zhou, J. P., Gu, Y. Q., Zou, C. S., & Mo, M. H. (2007). Phylogenetic diversity of bacteria in an earth-cave in Guizhou Province, Southwest of China. Journal of microbiology, 45(2), 105-112.

Evaluating the Inhibitory Effect  of  Streptomyces Bacteria against Pathogenic Bacteria and Study its Compatibility with Some Antibiotic Types



How to Cite

Risan, M. H., Subhi, S. A., & Hussain, B. . Y. (2022). Evaluating the Inhibitory Effect of Streptomyces Bacteria against Pathogenic Bacteria and Study its Compatibility with Some Antibiotic Types. Biomedicine and Chemical Sciences, 1(2), 35–40. https://doi.org/10.48112/bcs.v1i2.75