Fungal Survey of Different Clinical Samples Collected from Patients
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https://doi.org/10.5281/zenodo.15771842Abstract
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Candida is a genus of yeasts and is the most common cause of fungal infections worldwide. Candida spp., are eukaryotic representatives of the fungi kingdom. The genus Candida includes about 150 species, but many species are endosymbiosis of humans, causing infections mainly in immunosuppressed hosts. The frequency of Candida isolates as a cause of hospital infections has risen in recent years, leading to high rates of morbidity and mortality. The knowledge of the epidemiology of those hospital acquired fungal infections is essential to implement an adequate antifungal therapy. Fungal survey of different clinical samples collected from patients, to determine the effect of antifungal on C. albicans isolates from different body sites and lesions (blood, wounds swabs, ear swabs, bronchoalveolar lavage, high vaginal swabs, sputum, throat swabs, and urine). Swab sticks were collected from different sites of patients’ bodies (blood, wounds swabs, ear swabs, bronchoalveolar lavage, high vaginal swabs, sputum, throat swabs, and urine) then streaked directly on labelled Sabouraud's dextrose agar (SDA) plates and incubated at 25°C for (2-7) days. The growing colonies of Candida spp. isolates were initially diagnosed on (SDA) agar then confirmed by Chrome Candida Agar, the plates were incubated bunder aerobic conditions for 24 hr. at 37°C. Thereafter, antifungal sensitivity test was done. Only 209 of 1596 samples were taken from the patients and cultured on Sabouraud's dextrose agar were found to be positive. From 1596 just 59 isolate was Candida albicans after subculture on CROM Candida Agar, API candida and germ tube formation. Antifungal susceptibility tests were performed on 59 C. albicans isolates. Regarding antifungal resistance by C.albicans it was found 8 (14%) of isolates were resistant isolates resistant to fluconazole, 11 (19%) were resistant to miconazole as well as 11 (19%) were resistant to econazole, while 50 (85%) of isolates were sensitive to Nystatin, Clotrimazole (56, 95%), and to Ketoconazole (54, 92%). The study shows a higher antifungal resistance in the clinical samples which proves the risk in C. albicans management program.
Keywords:
Antifungal sensitivity test, C. albicans, Fungal surveyReferences
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