Design of a New Bioluminescence Sensor Using an Arduino Device Linked to Bluetooth Mobile Phone and USB Computer to Measure Triglycerides
DOI:
https://doi.org/10.48112/bcs.v2i2.410Abstract
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In this work, a new device is designed for measuring the light intensity of the Bioluminescence reaction between Luminol with Hydrogen peroxide that is produced as an activity of Triglycerides Phosphate Oxidase. The project includes the use of a homemade and semi-automated device for the determination of Chemiluminescence (CL) and Bioluminescence light by direct reaction analysis of Luminol with oxidant and using a Mobile –phone as a recorder which is employed in chemistry with Arduino and applied this device into the enzymatic reaction for determine Triglyceride by this new methods. The method is easy, simple, and rapid with high sensitivity for the determination of hydrogen peroxide. The light of the Bioluminescence reaction of Luminol (5-amino-2,3-dihydro-1,4_phthalazinedione) with oxidant (H2O2) was sensed by photocell and the signal was sent to Mobile. The method used to determine glycerol and compare the results of the device with real value to give high accuracy, Triglyceride was determined in its kit. It gave 99.2 and ±0.1 of Recovery and RSD, respectively.
Keywords:
Chemiluminescence, Bioluminescence, Luminol, hydrogen peroxide, TriglycerideReferences
Abow, R. A. M. (2017). Characterization, Functional Applications and Cytotoxicity of the Piliostigma reticulatum gum (Doctoral dissertation, Sudan University of Science and Technology). http://doi.repository.sustech.edu/handle/123456789/19760
Alizadeh, N., Salimi, A., & Hallaj, R. (2017). Hemin/G-Quadruplex horseradish peroxidase-mimicking DNAzyme: principle and biosensing application. Catalytically Active Nucleic Acids, 85-106. https://doi.org/10.1007/10_2017_37
Calabretta, M. M., Zangheri, M., Calabria, D., Lopreside, A., Montali, L., Marchegiani, E., ... & Michelini, E. (2021). Based Immunosensors with Bio-Chemiluminescence Detection. Sensors, 21(13), 4309. https://doi.org/10.3390/s21134309
Delafresnaye, L., Bloesser, F. R., Kockler, K. B., Schmitt, C. W., Irshadeen, I. M., & Barner‐Kowollik, C. (2020). All eyes on visible‐light peroxyoxalate chemiluminescence read‐out systems. Chemistry–A European Journal, 26(1), 114-127. https://doi.org/10.1002/chem.201904054
Fahliyani, S. A., Rastegari, A. A., Yadav, A. N., & Yadav, N. (2020). Microbially derived biosensors for diagnosis, monitoring, and epidemiology for future biomedicine systems. In New and Future Developments in Microbial Biotechnology and Bioengineering (pp. 43-65). Elsevier. https://doi.org/10.1016/B978-0-12-820528-0.00005-3
Guo, X., Hou, L. A., Yin, Y., Wu, J., Zhao, F., Xia, L., ... & Qiu, L. (2018). Negative interferences by calcium dobesilate in the detection of five serum analytes involving Trinder reaction-based assays. PloS one, 13(2), e0192440. https://doi.org/10.1371/journal.pone.0192440
Lee, J. K., Walker, K. L., Han, H. S., Kang, J., Prinz, F. B., Waymouth, R. M., ... & Zare, R. N. (2019). Spontaneous generation of hydrogen peroxide from aqueous microdroplets. Proceedings of the National Academy of Sciences, 116(39), 19294-19298. https://doi.org/10.1073/pnas.1911883116
Rahmawati, I., Saepudin, E., Fiorani, A., Einaga, Y., & Ivandini, T. A. (2022). Electrogenerated chemiluminescence of luminol at a boron-doped diamond electrode for the detection of hypochlorite. Analyst, 147(12), 2696-2702. https://doi.org/10.1039/D2AN00540A
Rambaldi, D. C., Reschiglian, P., Zattoni, A., & Johann, C. (2009). Enzymatic determination of cholesterol and triglycerides in serum lipoprotein profiles by asymmetrical flow field-flow fractionation with on-line, dual detection. Analytica chimica acta, 654(1), 64-70. https://doi.org/10.1016/j.aca.2009.06.016
Song, H., Meng, X., Wang, Z. J., Liu, H., & Ye, J. (2019). Solar-energy-mediated methane conversion. Joule, 3(7), 1606-1636. https://doi.org/10.1016/j.joule.2019.06.023
Vinegoni, C., Fumene Feruglio, P., Brand, C., Lee, S., Nibbs, A. E., Stapleton, S., ... & Weissleder, R. (2017). Measurement of drug-target engagement in live cells by two-photon fluorescence anisotropy imaging. Nature protocols, 12(7), 1472-1497. https://doi.org/10.1038/nprot.2017.043
Zhu, Y. C., Mei, L. P., Ruan, Y. F., Zhang, N., Zhao, W. W., Xu, J. J., & Chen, H. Y. (2019). Enzyme-based biosensors and their applications. In Advances in Enzyme Technology (pp. 201-223). Elsevier. https://doi.org/10.1016/B978-0-444-64114-4.00008-X
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