Immunological Study of Klebsiella Pneumoniae Isolated from Pneumoniae Infection Patients
AbstractAbstract Views: 244
This study aimed for isolation and identification of Klebsiella pneumoniae was an opportunistic pathogen responsible for a wide range of clinical syndromes such as pneumonia in both hospital and community settings. Assessment some immune parameter this study was carried out in Al-Diwaniya Teaching Hospital in Al-Diwaniya Province during the period from December 2018 to February 2019. A total of 272 individuals in both sex: 139 males and 133 females, including 210 sputum and blood specimens were collected from patients with pneumonia and 62 blood specimens were collected from healthy persons as a control group. The result of Microbiological tests of sputum was found 120(57.2%) specimens as Gram negative bacteria. From these specimens found 62(51.7%) have been appeared as positive result for K. pneumoniae and represented a major cause for pneumonia in this study, 37(59.7%) of them were males and 25(40.3%) female. 59(95.16%) of K. pneumoniae isolates have capsule when stained negatively with Indian ink. While, 60 (96.77%) isolates were appeared hypermucoviscosity (HVM) phenotype. The level of interferon gamma (IFN-γ) and toll like receptor (TLR4) concentration was measured by using enzyme-linked immunosorbent assay (ELIZA) and was found that IFN-γ is significantly (p ˂0.05) raised in all age groups of patients in comparison to the healthy control groups. The level of IFN-γ in age group (1-10) years recorded high percentage (293.123) compared with other age groups.. On the other hand, the level of TLR4 concentration was found that is significantly (p˂0.05) raised in all age groups of patients in comparison to the healthy control group. The level of TLR4 in age group (51-60) years recorded high percentage (12.993) compared to other age groups.
Keywords:TLR4, ELIZA, IFN-γ, K. pneumoniae
Ali, F. A., & Ismael, R. M. (2017). Dissemination of Klebsiella pneumonia and Klebsiella oxytoca Harboring bla TEM genes isolated from different clinical samples in Erbil City. Diyala Journal of Medicine, 12(2), 40-51.
Atlas, R. M., Brown, A. E., & Parks, L. C. (1995). Experimental microbiology: Laboratory manual. Mosby-Year Book, U.S.A. 1995;312.
Cai, X., Fu, Y., & Chen, Q. (2015). Association between TLR4 A299G polymorphism and pneumonia risk: a meta-analysis. Medical science monitor: international medical journal of experimental and clinical research, 21, 625.
Collee, J. G., Miles, R. S., & Watt, B. (1996). Tests for identification of bacteria. Mackie and McCartney practical medical microbiology, 14, 131-49.
Gharrah, M. M., Mostafa El-Mahdy, A., & Barwa, R. F. (2017). Association between virulence factors and extended spectrum beta-lactamase producing Klebsiella pneumoniae compared to nonproducing isolates. Interdisciplinary Perspectives on Infectious Diseases, 2017.
Hanada, S., Pirzadeh, M., Carver, K. Y., & Deng, J. C. (2018). Respiratory Viral Infection-Induced Microbiome Alterations and Secondary Bacterial Pneumonia. Frontiers in Immunology, 9, 2640-2640.
Harley, J. P., & Prescott, L. M. (1996). Laboratory exercises in microbiology. McGraw-Hill Science Engineering.
Hunt, J. J., Astley, R., Wheatley, N., Wang, J. T., & Callegan, M. C. (2014). TLR4 contributes to the host response to Klebsiella intraocular infection. Current eye research, 39(8), 790-802.
Ivin, M., Dumigan, A., de Vasconcelos, F. N., Ebner, F., Borroni, M., & Kavirayani, A. (2017). Natural killer cell-intrinsic type I IFN signaling controls Klebsiella pneumoniae growth during lung infection. PLoS Pathog, 13(11), e1006696.
Jeon, H. Y., Park, J. H., Park, J. I., Kim, J. Y., Seo, S. M., Ham, S. H., ... & Choi, Y. K. (2017). Cooperative interactions between Toll-like receptor 2 and Toll-like receptor 4 in murine Klebsiella pneumoniae infections. Journal of microbiology and biotechnology, 27(8), 1529-1538.
Lewis, S. M., Bain, B. J., & Bates, I. D. (2001). Lewis Practical Hematology. 9 [sup] th ed. London, UK: Churchill Livingstone.
MacFaddin, J. F. (2000). Biochemical tests for identification of medical bacteria. Lippincott Williams and Wilkins, USA.
Nirwati, H., Sinanjung, K., Fahrunissa, F., Wijaya, F., Napitupulu, S., Hati, V. P., ... & Nuryastuti, T. (2019, December). Biofilm formation and antibiotic resistance of Klebsiella pneumoniae isolated from clinical samples in a tertiary care hospital, Klaten, Indonesia. In BMC proceedings (Vol. 13, No. 11, pp. 1-8). BioMed Central.
Paczosa, M. K., & Mecsas, J. (2016). Klebsiella pneumoniae: going on the offense with a strong defense. Microbiology and Molecular Biology Reviews, 80(3), 629-661.
Parajuli, N. P., Acharya, S. P., Mishra, S. K., Parajuli, K., Rijal, B. P., & Pokhrel, B. M. (2017). High burden of antimicrobial resistance among gram negative bacteria causing healthcare associated infections in a critical care unit of Nepal. Antimicrobial Resistance & Infection Control, 6(1), 1-9.
Sakkas, H., Bozidis, P., Ilia, A., Mpekoulis, G., & Papadopoulou, C. (2019). Antimicrobial resistance in bacterial pathogens and detection of carbapenemases in Klebsiella pneumoniae isolates from hospital wastewater. Antibiotics, 8(3), 85.
Schüller, S. S., Sadeghi, K., Wisgrill, L., Dangl, A., Diesner, S. C., Prusa, A. R., ... & Förster‐Waldl, E. (2013). Preterm neonates display altered plasmacytoid dendritic cell function and morphology. Journal of leukocyte biology, 93(5), 781-788.
Sender, V., & Stamme, C. (2014). Lung cell-specific modulation of LPS-induced TLR4 receptor and adaptor localization. Communicative & integrative biology, 7(4), e29053.
van Lieshout, M. H., Florquin, S., vanʼt Veer, C., de Vos, A. F., & van der Poll, T. (2015). TIR-domain-containing adaptor-inducing interferon-β (TRIF) mediates antibacterial defense during gram-negative pneumonia by inducing Interferon-γ. Journal of innate immunity, 7(6), 637-646.
Vuotto, C., Longo, F., Balice, M. P., Donelli, G., & Varaldo, P. E. (2014). Antibiotic resistance related to biofilm formation in Klebsiella pneumoniae. Pathogens, 3(3), 743-758.
Xiong, H., Keith, J. W., Samilo, D. W., Carter, R. A., Leiner, I. M., & Pamer, E. G. (2016). Innate lymphocyte/Ly6Chi monocyte crosstalk promotes Klebsiella pneumoniae clearance. Cell, 165(3), 679-689.
How to Cite
Copyright (c) 2022 Biomedicine and Chemical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.