Molecular Identification of Four Eruca Sativa L. Cultivars using RAPD Markers
DOI:
https://doi.org/10.48112/bcs.v2i3.564Abstract
Abstract Views: 353
The current study was conducted at University of Kufa, Faculty of Science, Biology Department for molecular identification of four Eruca sativa L. cultivars (1-Turish 2-Syrian 3-Turkish 4-Eygyptian) using eleven Random Amplified Polymorphic DNA. Results indicate primers OPA-04, OPA-10, OPA-02, OPW-04, OPC-09,v and OPD-13 success in giving all cultivars a unique fingerprint .Highest number of main bands , polymorphic bands and discriminatory value were 20 , 18 and 20.22% respectively produced by OPA-02. Highest value for amplified band number and monomorphic bands were 44 and band produced by primers OPX-17 and OPD-13 respectively. Highest value for both polymorphism and Efficiency were 94.11% and 0.48 respectively produced by primer OPA-04. Genetic relationship and genetic distance among E.sativa cultivars distribution unrelated to their geographical origin. RAPD markers are efficient tool in studying E.sativa germplasm.
Keywords:
E.Sativa, Fingerprint , RAPDReferences
Abd El-Hady, E. A., Haiba, A. A., Abd El-Hamid, N. R., & Rizkalla, A. A. (2010). Phylogenetic diversity and relationships of some tomato varieties by electrophoretic protein and RAPD analysis. Journal of American Science, 6(11), 434-441.
Al-Qurainy, F., Alameri, A. A., & Khan, S. (2010). RAPD profile for the assessment of genotoxicity on a medicinal plant; Eruca sativa. Journal of Medicinal Plants Research, 4(7), 579-586.
Bahadur, B., Rajam, M. V., Sahijram, L., & Krishnamurthy, K. V. (Eds.). (2015). Plant biology and biotechnology. New Delhi: Springer.
Carelli, B. P., Gerald, L. T. S., Grazziotin, F. G., & Echeverrigaray, S. (2006). Genetic diversity among Brazilian cultivars and landraces of tomato Lycopersicon esculentum Mill. revealed by RAPD markers. Genetic Resources and Crop Evolution, 53, 395-400. https://doi.org/10.1007/s10722-004-0578-9
Deshwal, R. S., Singh, R., Malik, K., & Randhawa, G. J. (2005). Assessment of genetic diversity and genetic relationships among 29 populations of Azadirachta indica A. Juss. using RAPD markers. Genetic Resources and Crop Evolution, 52, 285-292. https://doi.org/10.1007/s10722-005-3282-5
Dhutmal, R. R., Mundhe, A. G., & More, A. W. (2018). Molecular marker techniques: A Review. International Journal of Current Microbiology and Applied Sciences, 6, 816-825.
El Nagar, M. M., & Mekawi, E. M. (2014). Comparison of different genotypes of rocket (Eruca sativa) in terms of chemical compounds extracted from seeds and in the callus induced from tissue culture. Middle East Journal of Agriculture Research, 3(4), 1074-1082.
El-Assal, S. E. D., & Gaber, A. (2012). Discrimination capacity of RAPD, ISSR and SSR markers and of their effectiveness in establishing genetic relationship and diversity among Egyptian and Saudi wheat cultivars. American Journal of Applied Sciences, 9(5), 724-735.
Ezekiel, C. N., Nwangburuka, C. C., Ajibade, O. A., & Odebode, A. C. (2011). Genetic diversity in 14 tomato (Lycopersicon esculentum Mill.) varieties in Nigerian markets by RAPD-PCR technique. African Journal of Biotechnology, 10(25), 4961-4967.
Graham, J., & McNicol, R. J. (1995). An examination of the ability of RAPD markers to determine the relationships within and between Rubus species. Theoretical and Applied Genetics, 90, 1128-1132. https://doi.org/10.1007/BF00222932
Hammer, O. (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontol Eletronica, 4, 9.
Hunter, P. R., & Gaston, M. A. (1988). Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. Journal of Clinical Microbiology, 26(11), 2465-2466. https://doi.org/10.1128/jcm.26.11.2465-2466.1988
Jaafar, N. S., & Jaafar, I. S. (2019). Eruca sativa Linn.: Pharmacognostical and pharmacological properties and pharmaceutical preparations. Asian Journal of Pharmaceutical and Clinical Research, 12(3), 39-45. https://doi.org/10.22159/ajpcr.2019.v12i3.30893
Kishore, L., Kaur, N., Kajal, A., & Singh, R. (2017). Extraction, characterization and evaluation of Eruca sativa against streptozotocin-induced diabetic nephropathy in rat. ||| Bangladesh Journal of Pharmacology|||, 12(2), 216-227. https://doi.org/10.3329/bjp.v12i2.32065
Nandani, K., & Thakur, S. K. (2014). Randomly amplified polymorphic DNA-a brief review. American Journal of Animal and Veterinary Sciences, 9(1), 6-13.
Nei, M., & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences, 76(10), 5269-5273. https://doi.org/10.1073/pnas.76.10.5269
Rahiman, F., Balasubramanian, T., Kumar, P., & Shejina, M. (2015). P-4 4 Random Amplified Polymorphic DNA (RAPD)-A tool for gene mapping. In National conference on Advances in Laboratory Medicine (Vol. 2, pp. 163-166).
Rao, V. R., & Hodgkin, T. (2002). Genetic diversity and conservation and utilization of plant genetic resources. Plant Cell, Tissue and Organ Culture, 68, 1-19. https://doi.org/10.1023/A:1013359015812
Sambrook, J., & Russell, D. W. (2001). Molecular Cloning: Ch. 8. In Vitro amplification of DNA by the polymerase chain reaction (Vol. 2). Cold Spring Harbor Laboratory Press.
Welsh, J., & McClelland, M. (1990). Fingerprinting genomes using PCR with arbitrary primers. Nucleic acids research, 18(24), 7213-7218. https://doi.org/10.1093/nar/18.24.7213
Williams, J. G., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic acids research, 18(22), 6531-6535. https://doi.org/10.1093/nar/18.22.6531
Zafar-Pashanezhad, M., Shahbazi, E., Golkar, P., & Shiran, B. (2020). Genetic variation of Eruca sativa L. genotypes revealed by agro-morphological traits and ISSR molecular markers. Industrial Crops and Products, 145, 111992. https://doi.org/10.1016/j.indcrop.2019.111992
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Biomedicine and Chemical Sciences
This work is licensed under a Creative Commons Attribution 4.0 International License.
