Factorial Design Experiment of the Impact of Heat Treatment on Milk Quality

Authors

Abstract

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The proposed study aimed to evaluate the influence of heat treatment temperature (65 ℃ and 75 ℃) and heating time (15s and 30s) on milk quality, to identify the optimal processing conditions that can guarantee microbial safety without compromising nutritional, physicochemical, and sensory characteristics. The experimental design was a 22 factorial design, and milk samples were tested at each combination of time and temperature under the control conditions. The quality parameters assessed were microbial count, pH, protein stability, colour, and taste. The results of the factorial ANOVA revealed that the temperature and heating time were both significant, and an interaction effect was observed as well, where the temperature effect is strongly affected by the heating time. The results of these studies prove that the factorial design is a powerful and efficient approach to the maximisation of the heat treatment parameters. The findings can be used to present evidence-based recommendations to dairy processors in order to ensure microbial safety without undermining the nutritional, physicochemical, and sensory properties of milk to facilitate better quality control/processing decisions in the dairy sector.

Keywords:

ANOVA, Dairy processing, Factorial design, Heat treatment, Microbial safety

References

Akram, M., Sami, M., Ahmed, O., Onyekere, P. F., & Egbuna, C. (2020). Health benefits of milk and milk products. In Functional foods and nutraceuticals: bioactive components, formulations and innovations (pp. 211-217). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-030-42319-3_12

Cimmino, F., Catapano, A., Petrella, L., Villano, I., Tudisco, R., & Cavaliere, G. (2023). Role of milk micronutrients in human health. Frontiers in Bioscience-Landmark, 28(2), 41. https://doi.org/10.31083/j.fbl2802041

Dash, K. K., Fayaz, U., Dar, A. H., Shams, R., Manzoor, S., Sundarsingh, A., ... & Khan, S. A. (2022). A comprehensive review on heat treatments and related impact on the quality and microbial safety of milk and milk-based products. Food Chemistry Advances, 1, 100041. https://doi.org/10.1016/j.focha.2022.100041

Faria Neto, A., Costa, A. F. B., & de Lima, M. F. (2018). Use of factorial designs and the response surface methodology to optimize a heat staking process. Experimental Techniques, 42(3), 319-331. https://doi.org/10.1007/s40799-017-0230-1

Fox, P. F., Uniacke-Lowe, T., McSweeney, P. L. H., & O’mahony, J. A. (2015). Heat-induced changes in milk. In Dairy chemistry and biochemistry (pp. 345-375). Cham: Springer International Publishing. https://doi.org/10.1007/978-3-319-14892-2_9

Fusco, V., Chieffi, D., Fanelli, F., Logrieco, A. F., Cho, G. S., Kabisch, J., ... & Franz, C. M. (2020). Microbial quality and safety of milk and milk products in the 21st century. Comprehensive Reviews in Food Science and Food Safety, 19(4), 2013-2049. https://doi.org/10.1111/1541-4337.12568

Giroux, H. J., Dupont, F., Villeneuve, G., & Britten, M. (2020). Effect of heating milk on whey protein denaturation and cheese-making properties. International Dairy Journal, 111, 104831. https://doi.org/10.1016/j.idairyj.2020.104831

Gunasekaran, S., & Yang, H. W. (2007). Effect of experimental parameters on temperature distribution during continuous and pulsed microwave heating. Journal of Food Engineering, 78(4), 1452-1456. https://doi.org/10.1016/j.jfoodeng.2006.01.017

Hafeez, K., Rowlands, H., Kanji, G., & Iqbal, S. (2002). Design optimization using ANOVA. Journal of Applied Statistics, 29(6), 895-906. https://doi.org/10.1080/02664760220136203

Kim, M. K., Jeong, S. G., Oh, M. H., Kim, D. H., Son, Y. S., & Ham, J. S. (2011). Guide to good dairy farming practice. Journal of Dairy Science and Biotechnology, 29(2), 75-80.

Lambrini, K., Aikaterini, F., Konstantinos, K., Christos, I., Ioanna, P. V., & Areti, T. (2021). Milk nutritional composition and its role in human health. Journal of Pharmacy and Pharmacology, 9, 8-13. https://doi.org/10.17265/2328-2150/2021.01.002

Ntuli, V., Sibanda, T., Elegbeleye, J. A., Mugadza, D. T., Seifu, E., & Buys, E. M. (2023). Dairy production: microbial safety of raw milk and processed milk products. In Present knowledge in food safety (pp. 439-454). Academic Press. https://doi.org/10.1016/B978-0-12-819470-6.00076-7

O’Connell, A., Ruegg, P. L., Jordan, K., O’brien, B., & Gleeson, D. (2016). The effect of storage temperature and duration on the microbial quality of bulk tank milk. Journal of Dairy Science, 99(5), 3367-3374. https://doi.org/10.3168/jds.2015-10495

Ortega, N., Albillos, S. M., & Busto, M. D. (2003). Application of factorial design and response surface methodology to the analysis of bovine caseins by capillary zone electrophoresis. Food Control, 14(5), 307-315. https://doi.org/10.1016/S0956-7135(02)00095-6

Osner, R. G., & Johnson, R. M. (1968). Nutritional changes in proteins during heat processing. International Journal of Food Science and Technology, 3(2), 81-86. https://doi.org/10.1111/j.1365-2621.1968.tb01441.x

Owusu-Kwarteng, J., Akabanda, F., Agyei, D., & Jespersen, L. (2020). Microbial safety of milk production and fermented dairy products in Africa. Microorganisms, 8(5), 752. https://doi.org/10.3390/microorganisms8050752

Ritota, M., Di Costanzo, M. G., Mattera, M., & Manzi, P. (2017). New trends for the evaluation of heat treatments of milk. Journal of Analytical Methods in Chemistry, 2017(1), 1864832. https://doi.org/10.1155/2017/1864832

Satpute, M., Annapure, U., & Marg, P. (2013). Approaches for delivery of heat sensitive nutrients through food systems for selection of appropriate processing techniques: a review. Journal of Hygienic Engineering and Design, 4, 71-92.

Singh, H., & Waungana, A. (2001). Influence of heat treatment of milk on cheesemaking properties. International Dairy Journal, 11(4-7), 543-551. https://doi.org/10.1016/S0958-6946(01)00085-1

Tsiafitsa, A., Oikonomopoulou, V., Stramarkou, M., Krokida, M., & Papassiopi, N. (2022). Effect of heat treatment on physicochemical and sensory properties of selected coffee varieties. European Food Research and Technology, 248(8), 2009-2020. https://doi.org/10.1007/s00217-022-04025-8

Walstra, P., Walstra, P., Wouters, J. T., & Geurts, T. J. (2005). Dairy science and technology. CRC press.

Wang, C., & Guo, M. (2019). Whey protein structure and denaturation and interactions with other food components. Whey Protein Production, Chemistry, Functionality, and Applications, 67-101. https://doi.org/10.1002/9781119256052.ch4

Published

2026-03-31

How to Cite

Naqvi, N., Zaidi, A. A., & Naqvi, S. (2026). Factorial Design Experiment of the Impact of Heat Treatment on Milk Quality. Bulletin of Multidisciplinary Studies, 3(1). Retrieved from https://journals.irapa.org/index.php/BMS/article/view/1215

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