Molecular Investigation of Salmonella spp from Broilers

Authors

  • Lobna Abdularaheem Shtaiwe Algburi Al-Furat Al-Awsat Technical University

DOI:

https://doi.org/10.48112/jestt.v1i1.672

Abstract

Abstract Views: 57

Salmonella sppare negative gram bacteria, rod-shaped; it belongs to Enterobacteriaceae family. Salmonella isa wide variety of species that causes many diseases in humans and animals. This investigation was carried out to study the molecular investigation of Salmonella spp from broilers. It was determined that approximately one hundred broilers were suffering from diarrhea, so feces and cloacal swabs were collected from them. These swabs were cultured on various media before sub-culturing on SS. agar and XLD. Following this, biochemical tests were performed to identify the bacteria. DNA extraction was carried out per the company's guidelines, and PCR was carried out to look for the invA gene, which is unique to Salmonella species. According to the findings, the percentage of individuals infected with Salmonella species was 17.39%. During this investigation, PCR was utilized to locate the invA gene, and out of the eight tested samples, Salmonella species were identified in 6 of them. Despite the severe and dangerous effects of the salmonella pandemic on the agricultural and economic sectors as well as on human health, the findings showed that the PCR method had a high specificity in detecting Salmonella sppwhen compared to other traditional methods of spotting the bacteria.

Keywords:

Broiler, Molecular, PCR, Salmonella

References

Humphrey, T. (2000). Public-health aspects of Salmonella infection. Salmonella in domestic animals, 1(1), 245-263. https://doi.org/10.1079/9780851992617.0245

Li, H., Wang, H., D'Aoust, J. Y., & Maurer, J. (2012). Salmonella species. Food microbiology: fundamentals and frontiers, 223-261. https://doi.org/10.1128/9781555818463.ch10

Afzal, A., Hussain, A., Irfan, M., & Malik, K. A. (2015). Molecular diagnostics for foodborne pathogen (Salmonella spp.) from poultry. Advancements in Life Sciences, 2(2), 91-97.

Eng, S. K., Pusparajah, P., Ab Mutalib, N. S., Ser, H. L., Chan, K. G., & Lee, L. H. (2015). Salmonella: a review on pathogenesis, epidemiology and antibiotic resistance. Frontiers in Life Science, 8(3), 284-293. https://doi.org/10.1080/21553769.2015.1051243

Herikstad, H., Motarjemi, Y., & Tauxe, R. (2002). Salmonella surveillance: a global survey of public health serotyping. Epidemiology & Infection, 129(1), 1-8. https://doi.org/10.1017/S0950268802006842

Schuurman, T., De Boer, R. F., Van Zanten, E., Van Slochteren, K. R., Scheper, H. R., Dijk-Alberts, B. G., ... & Kooistra-Smid, A. M. D. (2007). Feasibility of a molecular screening method for detection of Salmonella enterica and Campylobacter jejuni in a routine community-based clinical microbiology laboratory. Journal of clinical microbiology, 45(11), 3692-3700. https://doi.org/10.1128/jcm.00896-07

Gómez-Duarte, O. G., Bai, J., & Newell, E. (2009). Detection of Escherichia coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Vibrio cholerae, and Campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction. Diagnostic Microbiology and Infectious Disease, 63(1), 1-9. https://doi.org/10.1016/j.diagmicrobio.2008.09.006

Pilla, G., & Tang, C. M. (2018). Going around in circles: virulence plasmids in enteric pathogens. Nature Reviews Microbiology, 16(8), 484-495. https://doi.org/10.1038/s41579-018-0031-2

El-Sebay, N. A., Abu Shady, H. M., El-Rashed El-Zeedy, S., & Samy, A. (2017). InvA gene sequencing of Salmonella Typhimurium isolated from Egyptian poultry. Asian J. Sci. Res, 10(3), 194-202.

Ferretti, R., Mannazzu, I., Cocolin, L., Comi, G., & Clementi, F. (2001). Twelve-hour PCR-based method for detection of Salmonella spp. in food. Applied and Environmental Microbiology, 67(2), 977-978. https://doi.org/10.1128/AEM.67.2.977-978.2001

Jellbauer, S., Perez Lopez, A., Behnsen, J., Gao, N., Nguyen, T., Murphy, C., ... & Raffatellu, M. (2016). Beneficial effects of sodium phenylbutyrate administration during infection with Salmonella enterica serovar Typhimurium. Infection and immunity, 84(9), 2639-2652. https://doi.org/10.1128/iai.00132-16

Jawad, A. A. (2010). Detection of fim A, rfbj (B) and fimC Genes of Salmonella Isolates Using Singleplex and Multiplex Polymerase Chain Reaction (Doctoral dissertation, M. Sc. Thesis, College of Medicine, Al-Qadisiya University).

Al–Janabi, J. K. (2006). Microbiological study of some causative agents of diarrhea in children under five year of age in Al–Diwaniya city (Doctoral dissertation, Ph. D. Thesis. College of Education, Al-Qadisiya University).

Van Dijk, S., Bruins, M. J., & Ruijs, G. J. (2009). Evaluation and implementation of a chromogenic agar medium for Salmonella detection in stool in routine laboratory diagnostics. Journal of Clinical Microbiology, 47(2), 456-458. https://doi.org/10.1128/jcm.01643-08

Perez, J. M., Cavalli, P., Roure, C., Renac, R., Gille, Y., & Freydiere, A. M. (2003). Comparison of four chromogenic media and Hektoen agar for detection and presumptive identification of Salmonella strains in human stools. Journal of clinical microbiology, 41(3), 1130-1134. https://doi.org/10.1128/jcm.41.3.1130-1134.2003

Cohen, H. J., Mechanda, S. M., & Lin, W. (1996). PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp. Applied and Environmental Microbiology, 62(12), 4303-4308. https://doi.org/10.1128/aem.62.12.4303-4308.1996

Drahovská, H., Turňa, J., Piknová, Ľ., Kuchta, T., Szitasová, I., Škarková, A., & Sásik, M. (2001). Detection of Salmonella by polymerase chain reaction targeted to fimC gene. Biologia, Bratislava, 56(5), 607-612.

Pathmanathan, S. G., Cardona-Castro, N., Sánchez-Jiménez, M. M., Correa-Ochoa, M. M., Puthucheary, S. D., & Thong, K. L. (2003). Simple and rapid detection of Salmonella strains by direct PCR amplification of the hilA gene. Journal of Medical Microbiology, 52(9), 773-776. https://doi.org/10.1099/jmm.0.05188-0

Abdelaziz, I. S., El-Tawab, A., Awad, A., Maarouf, A. A., & Elhofy, F. I. (2020). Bacteriological and molecular studies on Salmonella isolated from duckling farms at Kaliobia, Egypt. Benha Veterinary Medical Journal, 39(1), 169-174. https://dx.doi.org/10.21608/bvmj.2020.38777.1242

Al-Kaaby, K. T., Al-Dabhaw, A. H., & Samaka, H. M. (2014). Detection of Some SalmonellaEnteritidisVirulence Genes by Multiplex-PCR Assay Using Two Different DNA Extraction Methods. Al-Qadisiyah Medical Journal, 10(18), 63-70.

Hussain, S. S., Mezal, E. H., & Al-yasiri, M. H. (2019). Isolation and Antibiogram of Salmonella enterica from Children Under Five Years with Diarrhea in Thi-Qar Province. Journal of Education for Pure Science, 9(1).

Published

2024-02-29

How to Cite

Algburi, L. A. S. (2024). Molecular Investigation of Salmonella spp from Broilers. Journal of Engineering, Science and Technological Trends, 1(1), 1–6. https://doi.org/10.48112/jestt.v1i1.672

Issue

Section

Articles